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 Results and Conclusion

gram-column-natural-starches

Three different types of starches were separated on PSS GRAM columns with DMSO, 5 g/L LiBr as mobile phase. Calibration with polysaccharides (pullulans) allows the relative determination of the molar mass distributions (Figure 1).

A light-scattering detector was used to confirm a successful sample preparation and the achievement of true SEC/GPC separation. Light-scattering detectors are molar mass sensitive detectors. These detectors are ideal for high molecular weight samples and would detect non-dissolved particles with high sensitivity. Additionally they can measure the molar mass directly, if the refractive index increment, dn/dc, is known. For high molar masses large light scattering signals are expected, for low molar masses small signals are expected.

Figure 2 shows the chromatogram of potato starch with the light scattering and refractive index signal. This figure shows that the separation on the GRAM columns is based on true SEC/GPC mode: the large molecules elute first and show a large light scattering signal, the smaller molecules elute at the end and show a small signal. Sample preparation has been successful since no non-dissolved particles can be detected by the light scattering detector. Whereas, sample decomposition can be excluded because the refractive index detector does not show signal in the low molecular weight region after the main peak.

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