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Column Types and Applications

GPC Column Types

  

Column type

Column dimension
I.D.* x length [mm]**

Field of application

Precolumn

  • analytical
    8 x 50
  • micro
    4.6 x 30
  • preparative
    20 x 50

Protection of separation columns

Analytical

8 x 300

GPC/SEC analysis

Micro

4.6 x 250

GPC/SEC analysis optimized for solvent saving and small sample amounts

Preparative

20 x 300
20 x 600
40 x 250

Preparative sample fractionation, sample collection with GPC/SEC

HighSpeed

20 x 50

Ultra fast GPC/SEC analysis

Lux

8 x 300

Optimized for GPC/SEC-Light Scattering

* I.D.: internal diameter
** Any other column dimension on request

 

Column particle size

Particle Size through GPC Column technology

PSS GPC/SEC column materials are available in several particle sizes from 3 μm up to 20 μm particles.

 

The optimum particle size for a given application depends on

 

  • the solvent viscosity: 
    higher viscosity requires higher particle size
  • the sample molar mass: 
    higher molar masses require higher particle sizes.

 

 


Porosity

The pore size distribution of the column material determines the separation range.

  • To separate low molecular weight products use small porosities.
  • The higher the molecular weight the higher is the required porosity.

 

   

Single porosity columns show a high resolution in a narrow molecular weight range. A wide molecular weight range at constant high resolution is covered if columns with single porosities are coupled in series (column combination).

 

Examples for single porosity columns:

  • SDV 1 000 Å, separation range: 100 - 60 000 Da 
  • GRAM 3 000 Å, separation range: 5 000 - 5 000 000 Da
  • SUPREMA 1 000 Å, separation range: 100 - 1 000 000 Da

 

Examples for recommended column combinations (series of single porosity columns):

  • SDV 100 Å + 1 000 Å + 10 000Å + precolumn (low molar masses)
  • GRAM 30 Å + 1 000 Å + 1 000Å + precolumn (medium molar masses)
  • SUPREMA 100 Å + 3 000 Å + 3 000Å + precolumn (high molar masses)

 

   

Linear or mixed bed columns provide a very wide porosity range therefore offering a broad separation range with a smaller resolution.

 

Examples for linear columns:

  • SDV linear M separation range: 100 - 1 000 000 Da 
  • GRAM linear, separation range: 500 - 1 000 000 Da
  • SUPREMA linear XL, separation range: 1 000 - 4 000 000 Da

 

 

 

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