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Columns

Columns for Macromolecular Liquid Chromatography: Gel Filtration, Gel Permeation, Size Exclusion Chromatography 

Our stationary phases designed for Interaction Free GPC/SEC come in stainless steel columns of standard dimensions that fit any HPLC or LC (GPC, SEC, GFC) instrument.

 

Click picture for close up view    Column Selection Guide
Facts and Tips

Magic Triangle for Aqueous GPC
  Columns for polymers soluble in water + salts/buffers, MeOH, acetonitrile 
Suprema         pH 2 - 12 
Proteema        pH < 7  for proteins
Novema          TFA, pH 1.5 -13
MCX               pH 1 - 14

Magic Triangle for Organic GPC
 

Columns for polymers soluble in Organic Solvents
SDV        THF, Toluene, Chloroform and Methylene chloride
GRAM      for DMF, DMAC, NMP, DMSO Specifications   
POLEFIN  for TCB, ODCB, Decalin
PFG Columns 



Lux Columns
  Columns for Light Scattering Detection
SDVLux 
SupremaLux 
POLEFINLux 
Lux columns reach the steady state mode > 25 times faster than conventional columns, and their overall noise reduction is more than 25 times better.
Traditional GPC/SEC columns can produce excessive baseline noise from particulates in the eluent stream, which can significantly deteriorate the quality of the light scattering detectors data (especially LALLS/LALS)  Normally a new column set needs a 'conditioning' process, which involves flushing the columns to waste without any detector connected.

One linear or Multiple columns
  Mixed Bed Column vs. Column Combination
Comparison linear column XL with a column combination for high molar masses
One Linear Column: fast analysis time, broad molar mass range 
Column combination: high resolution



analytical vs Highspeed
HighSpeed Calibration

 
HighSpeed vs. Analytical Columns 

A HighSpeed column (red line) reduces the run time for a sample (broad Poly(styrene)) by a factor of 6 - 8 compared to a conventional column (black line).

HS will substitute for analytical columns without modifications to your LC system, as long as pump and detectors accommodate 6.25 ml/min. At this flow rate, you will speed up the measurement using the same amount of solvent you currently use.

Calibration with Poly(styrene) ReadyCal green, red, and white: each contains 4 different molecular weight standards

PSS offers a wide variety of column dimensions and will manufacture custom sizes upon request. 
 
Type                      I.D x Length (mm)   
Guard columns        8 x 50; 4,6 x 30; 20 x 50         
Analytical                8 x 300 and 8 x 600   
Microbore                4,6 x 250   
Preparative              20 x 600   
HighSpeed               20 x 50 

Browse our entire selection of columns here.

Analytical, HighSpeed and guard Columns may be purchased online.  Email us or Request a Quotefor more information on analytical columns (8x600), Microbore and Preparative columns

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Polymer Standards

Molecular Weight Reference Polymer Standards

PSS manufacturers standard reference polymers for applications in aqueous and organic solvents.  The
wide variety of calibration standards are suitable for different applications and come individually or assembled into Kits, carefully selected to cover given molecular weight ranges suitable for GPC, SEC, GFC calibration.  

For testing in Organic Solvent Environments       

For testing in Aqueous (water) Environments 
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Oligo(ethylene) monodisperse 
Poly(2-vinylpyridine) 
Poly(alpha-methylstyrene)
Poly(butadiene-1.4)
Poly(carbonate) 
Poly(dimethylsiloxane) 
Poly(ethyl methacrylate) 
Poly(ethylene terephthalate) 
Poly(ethylene) 
Poly(isobutylene) 
Poly(isoprene-1.4) 
PolyLactide Kit
Poly(methylmethacrylate) 
Poly(n-butyl methacrylate) 
Poly(para-methylstyrene) 
Poly(propylene glycol) 
Poly(t-butyl acrylate) 
Poly(t-butyl methacrylate) 
Polystyrene

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Dextran 
Hydroxy-propyl Cellulose 
Poly(2-vinylpyridinium bromide) 
Poly(acrylamide) 
Poly(acrylic acid) Na Salt 
Poly(vinyl alcohol) broad 
Polyethylene glycol 
Polyethylene Oxide 
Polystyrene Sulfonate Sodium Salt 
Poly(methacrylic acid) Sodium Salt
Poly(DADMAC)
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Testing Lab for Analytical Services

Testing Laboratory for Macromolecular Analytical Services

 


At PSS analytical services division we address each sample with the highest of priority in adherence to our DIN EN ISO 9001 certification and standard methodologies.  PSS provides polymer characterization in a variety of solvents (THF,  Toluene, DMF, DMAC, NMP, TCM, HFIP, TFE , Water,  DMSO and TCB) at temperatures up to 155°C.
 
We routinely run thousands of customer samples each year using various standard methodologies, at customers request or implementing the client's conditions:

The following links take you to a brief description of the technique and online ordering Additional reading
High Temperature GPC Advanced Polyolefin Analysis
Conventional Gel Permeation Chromatography -GPC/SEC/GFC with UV, RI, ELSD, Detection Molar Mass Determination.
FTIR Coupling withGPC/SEC/GFC
 
GPC/SEC/GFC Light Scattering Coupling 
GPC/SEC/GFC Viscometry Coupling
Structure Elucidation with Molar Mass Sensitive Detectors
Refractive Index Increment 
Batch Light Scattering 
Vapour Pressure Osmometry
 


Triple Detection using viscosity, light scattering and concentration detectors(UV, RI, ELSD) as well as  Other physical characterization, methods e.g. end group analysis by NMR, MALDI - TOF - MS,  pyrolysis GC, DSC, dynamic light scattering etc., are available upon request 


Confidentiality Agreement

If you have confidentiality requirements, we routinely deal with this kind of situations.  Just email your agreement requirement and we will promptly process it. Contact us for more information.


Ordering Laboratory Services


  • Fill out the Sample Testing Evaluation Form.
  • Place the order 
  • Ship the samples, the Sample Testing Evaluation Form and (if available) the sample MSDS to:                                                                                                         Polymer Standards Services USA, Inc
                           Amherst Fields Research Park
                           160 Old Farm Rd, Suite A
                           Amherst, MA 01002 
  • Receive your results via e-mail, 10 days from PSS receipt of your sample 
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PSS USA

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Columns FAQ

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Instruments

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Software

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GPC Training

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News

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Blog

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Tips and Tricks

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Archived News

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Latest News

  • Introducing our new Online Store +

    We are pleased to announce that we will be launching our NEW and IMPROVED PSS online store on November 2nd . You Read More
  • GPC/SEC SYMPOSIUM 2017 +

    October 2-5, 2017 New Orleans, Louisiana The International Symposium on GPC/SEC and Related Techniques is designed for users from all backgrounds Read More
  • Liquid Adsorption Chromatography Seminar April 2017 +

    PSS USA Inc. will be presenting a seminar on Liquid Adsorption Chromatography in Amherst, MA on April 11th, 2017.   This course Read More
  • Speaking at 16th Annual Csaba Horvath Memorial Award Symposium +

    Dr. Derek Lohmnn will be speaking at the 16th Annual Csaba Horvath Memorial Award Symposium on the Yale West Campus in Read More
  • Pullulan ReadyCal Kits for GPC/SEC calibration +

    The calibration of a GPC/SEC-system is a time-consuming process as it requires the accurate weighing of a larger number of different Read More
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