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Protein Aggregation with Gel Chromatography 

Protein aggregation is a very common property of polypeptide chain occurring from  interactions with solvent and with neighbor molecules.  The aggregation of proteins into insoluble intracellular complexes is a common problem in bioengineering; in particular, it is becoming a problem of significant economic importance as shown by studies on the production of therapeutic proteins and their use as possible drugs in some neurodegenerative disorders. (M. Leone)

PSS will not attempt to provide pathways or explanations of the aggregation mechanism, but  tools to ease the separation/ad light scattering properties of aggregates.  

Separation of BSA Protein Aggregates by size exclusion  Chromatography 
Order PROTEEMA columns for Light scattering detection

Proteema gel is a stable and robust silica material well suited for use with light scattering detectors. Its exceptional mismatch-free performance is due to the highly controlled pore size distribution of the silica phase that has been chemically treated with a perfect supply of functional groups;  


Aggregation testing service
You can use our Contract Laboratory services to  perform the aggregation testing of your sample contact us for a quote.


Other Columns for Gel Chromatography of polymers soluble in water 
Suprema         water + salts/buffers, MeOH, acetonitrile, pH 2 - 12 
SupremaLux    Light Scattering in water + salts/buffers, MeOH, acetonitrile, pH 2 - 12 
Novema           water + salts/buffers, MeOH, acetonitrile, TFA, pH 1.5 -13
MCX                water + salts/buffers, MeOH, acetonitrile, pH 1 - 14

Analytical, HighSpeed and guard Columns may be purchased online. Contact us or Request a Quote for a custom quote on analytical columns (8x600), Microbore and Preparative columns.

 

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