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  • Purge the system with mobile phase; pump solvent through to remove air from the capillary to prevent air from entering the column; flush the injection loop also.
  • Use the column connections supplied to connect columns in series
  • Use a middle porosity as the first column use the highest porosity as the last
  • Be aware of column flow direction  (operating the flow rate in the reverse direction is only part of troubleshooting or operating after a long storage time)
  • Thread the column fittings finger tight into the system (do not over tighten the fitting! Over-tightening will  damage the column and the column head)
  • Do not connect the column to the detector during the installation and the first solvent exchange
  • Flush the column with 10 times of the column volume at 1/5 of the recommended flow rate: 0.2 ml/min for ID 8 mm
  • When the solvent exchange is completed, connect to the detector
  • Slowly increase the flow rate to the typical operating flow rate which depends on the column dimensions (see table below) 

When installing multiple columns:

  • Check each column separately as described above
  • Check the combined plate count for the column series after installation
  • Install the largest porosity in front of the combination
  • Install the smallest porosity in the middle and  the medium porosity at the end and
  • Use only recommended column combinations
  • Do not combine linear and single porosity columns

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