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Q: How do I store my Columns?


A: Storage Recomendations
  1. Remove all salt solutions with pure solvents and plug tight with the original end plugs.
  2. Store the columns in the solvent used during shipment;  If the column was shipped and used in THF or CHCl3 you can store the column in toluene as well. Toluene has the advantage of a very low evaporation  rate. Columns shipped in DMAc or DMF must be stored in DMAc or DMF.
  3. It is good practice to keep columns with volatile mobile phases in a refrigerator (4°C) after use to prevent  solvent evaporation.  Warning never let the column temperature fall below the freezing point of the storage solvent. This can destroy the stationary phase.
  4. Occasionally solvent is lost during long-term storage or due to high storage temperatures. When the expected pressure does not build up or the pump constantly re-regulates the flow, it is an indication of a partially evaporated column solvent.  
Recovering a partially dry column

To re-wet a partially dry column, install it in the reverse direction (to prevent the packing from damage).  Using a flow of 0.1 ml/min, fill the column until no more bubbles appear at the column outlet. Switch the column to the correct flow direction and again use 0.1 ml/min for 1h. Then you can slowly increment the flow rate, systematically, using 0.2 ml/min steps.

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