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Aqueous Gel Filtration Chromatography

PSS produces many water soluble, highly characterized molecular weight standards to calibrate the Gel Filtration Chromatography measurements, given that both molecular weight and three dimensional shape contribute to the degree of retention.

The following  polymer standards are available for aqueous applications


Dextran
Hydroxyethyl Starch
Poly(2-vinylpyridinium bromide)
Poly(acrylamide) Broad
Poly(acrylic acid) Na Salt
Polyethylene glycol
Poly(DADMAC)
Polyethylene Oxide
Poly(methacrylic acid) Sodium Salt
Polystyrene Sulfonate Sodium Salt
Poly(vinyl alcohol)
Protein
Pullulan

Polyethylene glycol is commonly used as a calibrant in aqueous applications.  It is also  used as a reactive ingredient for PEGylation, an approach for protein modification (preparation of protein-PEG conjugates). 
Covalent attachment of poly(ethylene glycol) (PEG) to proteins produces conjugates with altered/improved physicochemical and biological properties which depend upon the number of PEG chains linked.(*)  Delgado, et. al used Dextran (polysaccharide standard)  in conjunction with PEG to assist in the quantitative analysis pf PEG in PEG-modified proteins

PSS produces three different stationary phases for GFC, that are packed in columns suitable for any commercial liquid chromatograph. GFCis used in Biotechnology applications (proteins, peptides, oligonucleotides. etc),  for analysis of molecular size, for separations of components in a mixture, or for salt removal or buffer exchange from a preparation of marcromolecules.

*  (Delgado, et. al.  Quantitative analysis of polyethylene glycol (PEG) in PEG-modified proteins/cytokines by aqueous two-phase systems Journal of biochemical and biophysical methods, 1994 Dec;29(3-4):237-50.

 

 

 

 

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