Aqueous Gel Filtration Chromatography
PSS produces many water soluble, highly characterized molecular weight standards to calibrate the Gel Filtration Chromatography measurements, given that both molecular weight and three dimensional shape contribute to the degree of retention.
The following polymer standards are available for aqueous applications
Poly(acrylic acid) Na Salt
Poly(methacrylic acid) Sodium Salt
Polystyrene Sulfonate Sodium Salt
Polyethylene glycol is commonly used as a calibrant in aqueous applications. It is also used as a reactive ingredient for PEGylation, an approach for protein modification (preparation of protein-PEG conjugates).
Covalent attachment of poly(ethylene glycol) (PEG) to proteins produces conjugates with altered/improved physicochemical and biological properties which depend upon the number of PEG chains linked.(*) Delgado, et. al used Dextran (polysaccharide standard) in conjunction with PEG to assist in the quantitative analysis pf PEG in PEG-modified proteins
PSS produces three different stationary phases for GFC, that are packed in columns suitable for any commercial liquid chromatograph. GFCis used in Biotechnology applications (proteins, peptides, oligonucleotides. etc), for analysis of molecular size, for separations of components in a mixture, or for salt removal or buffer exchange from a preparation of marcromolecules.
* (Delgado, et. al. Quantitative analysis of polyethylene glycol (PEG) in PEG-modified proteins/cytokines by aqueous two-phase systems Journal of biochemical and biophysical methods, 1994 Dec;29(3-4):237-50.